Innovation Newsletter

The MUSIQ network shares updates and highlights on the project’s progress, results and ESR’s activities, with a focus on the latest innovation related to multiphoton microscopy and bioimaging through dedicated online Innovation Newsletters.

MUSIQ Innovation Newsletter n. 5 | November 2022

Ultrafast Optics in Microscopy: Recent Advances

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3D Imaging with Femtosecond Pulses
Combining 3D Imaging with Ultrafast Optics

The 3D imaging technique of Optical Diffraction Tomography is intrinsically ill-suited for ultrafast laser pulses. However, it could be realized using standard optical equipment with femtosecond pulses. This opens up new possibilities in cell-imaging and ultrafast material studies.  Read more

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From 3D to Polarisation to Single Nanoparticle Microscopy
Probing Biological Molecular Organization with Polarized Light Microscopy

Using light’s polarization can help illuminate the organization of biological molecules without perturbing the sample itself. Thus, polarization microscopy adds a new dimension to light microscopy for better understanding of biomedical questions. Read more

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Spectroscopy in wide field imaging
Imaging for spectroscopy

In this section we introduce the concept of Fourier transform spectroscopy (FTS). The technique is fast compared to point scaning of single nanoparticles. This section explains how which just recorded image set one can retire the spectral information of nanoparticles and molecules. Read more

MUSIQ Innovation Newsletter n. 4 | August 2022

EBL and FIB lithography to produce plasmonic single nanostructures or arrays on support

Thanks to their optical properties, including the ability to confine light in spatial regions below the diffraction limit, plasmonic nanostructures have found important applications in many different fields. Read more

OPTO NANO – A duet of Science and Contemporary Dance

Three of our ESRs, usually focussed on analysing the electronic response of metallic or semiconducting nanostructures to electromagnetic waves, were invited to change their perspective on their scientific and oftentimes highly conceptual work and translate their science into choreographed movement. Read more

MUSIQ Innovation Newsletter n. 3 | October 2021

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Prospects of stimulated Raman scattering in pharma research

Before a novel medicine can be introduced on the market, it needs to undergo a drug development process which today on average consists of six stages. The drug development process from target discovery to the launch can  take up to 15 years. Read more

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Light sources for CARS microscopy

In coherent anti-Stokes Raman scattering (CARS) microscopy two laser beams are used to produce radiation at a third wavelength when energy difference between pulses matches vibrational energy of a sample. For this vibrationally specific microscopy method it is critical to have a reliable wavelength-tunable light source to address different Raman bands. Optical parametric amplifiers (OPAs) and oscillators (OPOs) are well suited for this task. Read more

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EMCCD and sCMOS cameras for scientific imaging
A comparison of the key figures of current camera models

The emergence of faster and more sensitive scientific cameras allows to acquire images with unprecedented sensitivity and speed. This is particularly important for applications in life sciences where techniques like Raman imaging require low-noise detectors while other techniques like fluorescent correlation spectroscopy rely on frame rates in the upper kilohertz range. To obtain meaningful data it is therefore essential to choose the scientific camera according to the experimental conditions.  Read more

MUSIQ Innovation Newsletter n. 2 | March 2021

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Broadband laser sources for ultrafast spectroscopy

The continuous progress in laser technology along with technical advances and conceptual breakthroughs, expand the application potential of Ultrafast Spectroscopy. Read more

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Pulse shaping techniques for ultrafast spectroscopy

This section focuses on programmable femtosecond pulse-shaping techniques for multidimensional ultrafast spectroscopy. Read more

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Resources for improving data acquisit/newsletter/ion and coherence characterization in ultrafast spectroscopy

This section focuses on: 1. eliminating heat-induced signals by controlling pulse spectra through pulse shaping, 2. compressive sensing to shorten the data acquisition time, and 3. simultaneous frequency and time resolution using time-frequency transforms.  Read more

MUSIQ Innovation Newsletter n. 1 | October 2020

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Laser sources for Coherent Raman Scattering Microscopy

State of the art and new technologies – How to choose the best laser source for your SRS experiments

The laser source is arguably the core component in any CRS experiment. In this brief article we explain what the most common laser sources for CRS microscopy are and try to give guidelines for their selection. Read more

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Light sources for harmonic generation and multiphoton fluorescence microscopy

This section informs about the latest laser sources and their requirements for biophotonics applications. Specifically, the field of multiphoton microscopy (MPM) was subject to vast developments in recent years, pushed by the development of pulsed laser sources. This article will provide an overview of specific requirements of MPM experiments, and therein requirements for the laser sources themselves. Read more